Bafana are scheduled to welcome Ghana to FNB Stadium in Johannesburg on 25 March before going away to Omdurman to face Sudan at El Hilal Stadium three days later.
South Africa are currently second in Group C, level on points with Ghana but one goal behind in goal difference, after four games played in the campaign.
Sudan, with 6 points and a fixture against Sao Tome and Principe to play, are the only threat to their spots at next year’s continental championships, which is set to take place in Cameroon from January to February.
Ntseki has retained veteran shot stopper Itumeleng Khune, despite his poor form for Kaizer Cheifs of late, while handing a maiden call-up to AmaZulu goalkeeper Veli Mothwa.
The home match against Ghana and the away leg to Sudan are the final two matches for South Africa in their bid to qualify for the Afcon, to be hosted in Cameroon next year. Only two teams from the group can book a place at the Afcon.
Due to the Covid-19 pandemic, the tournament was moved from taking place this year and has now been rescheduled for January 2022.
Below is the full list:
Goalkeepers: Veli Mothwa (AmaZulu FC), Ronwen Williams (SuperSport United FC), Itumeleng Khune (Kaizer Chiefs FC)
Defenders: Thibang Phete (OS Belenenses, Portugal), Siyanda Xulu (Haopel Tel Aviv, Israel), Thulani Hlatshwayo (Orlando Pirates), Mosa Lebusa (Sundowns), Innocent Maela (Orlando Pirates), Sifiso Hlanti (Swallows), Thapelo Morena (Sundowns), Craig Martin (Cape Town City)
Midfielders: Rivaldo Coetzee (Sundowns), Themba Zwane (Sundowns), Thulani Serero (Al Jazira FC, Abu Dhabi), Ben Motshwari (Orlando Pirates), Bongani Zungu (Rangers FC, Scotland), Keagan Dolly (Montpellier FC, France), Percy Tau (Brighton and Hove Albion, England), Sipho Mbule (Supersport United), Andile Jali (Sundowns), Luther Singh (Paços de Ferreira,Portugal), Dean Furman (Carlisle United FC, England)
Forwards: Kermit Erasmus (Sundowns FC), Bradley Grobler (Supersport United), Ruzaigh Gamildien (Swallows FC), Lyle Foster (Victoria Guimaraes, Portugal)